Researchers from Xi’an University of Architecture and Technology, et al. have conducted a study entitled “Biochar mediated microbial synergy in Partial nitrification–anammox systems: enhancing nitrogen removal efficiency and stability”. This study was published in Frontiers of Environmental Science & Engineering, Volume 19, Issue 7.

This study investigated the role of biochar in enhancing nitrogen removal efficiency (NRE) and stability in partial nitrification–anammox (PN/A) systems, focusing on its selective promotion of partial denitrification and maintaining the dynamic balance between AnAOB and denitrifying bacteria (DNB).
READ MORE: Scientists boost denitrification of wastewater with biochar-based substrate
The results showed that biochar enhanced electron transfer, effectively reduced nitrate accumulation and significantly improved PN/A system NRE and stability.
High ammonium conditions
Under high ammonium conditions (800 mg/L), biochar increased NRE in the PN/A system to 83.58%, a 10% improvement over the anammox-control (ACK). Additionally, partial denitrification contributed 8% more to total nitrogen removal in the anammox-biochar (ABC) system.
The porous structure and redox-active groups of biochar provided an ideal environment for key microorganisms, promoted microbial growth and increased specific anammox activity by approximately 1.25–1.46 times compared to ACK, further enhancing microbial stability under fluctuating nitrogen loads.

Biochar also enriched AnAOB and DNB communities, sustained their dynamic balance and improved system stability by promoting nitrogen removal related gene expression. Overall, biochar demonstrated great potential for improving PN/A system efficiency, optimizing wastewater treatment, and reducing energy consumption and emissions.
For more detailed information, the full paper is available at: https://journal.hep.com.cn/fese/EN/10.1007/s11783-025-2015-6.
	
                
                
                
                
                
                
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