Scientists have created a 3D model of Helicobacter pylori to better understand its movement, hoping to crack the code governing the organism’s motility and develop alternative treatments for infections, such as strengthening the gastric mucus barrier that stands against the bacteria.
The spiral-shaped microorganism uses its corkscrew-like tail to power forward through viscous fluids such as stomach mucus. When it arrives at the epithelium of the stomach wall, it can cause everything from ulcers to cancer.
In a new study published by Physical Review Letters, FAMU-FSU College of Engineering researchers created a 3D model of this bacteria to better understand its movement.
“People around the world have treated ulcers with antibiotics because antibiotics kill bacteria, but it’s a double-edged sword,” said study co-author Hadi Mohammadigoushki, an associate professor in the Department of Chemical and Biomedical Engineering. “If we understand how these bacteria move, we can work toward providing other solutions for treatment.”
High viscosity gel
In the experiments, the team placed a model of the bacteria in a high-viscosity polymer gel, an example of what’s called a yield-stress fluid. Those fluids behave as solids under small stresses but flow like liquids beyond a critical stress point.
Then they used a magnetic field to rotate the 3D model, mimicking the behavior of the microorganism. Using particle tracking and imaging techniques, the researchers measured the speed of the bacteria and visualized the distribution and density of the fluid flowing around it.
The researchers identified two critical thresholds that the bacteria must overcome: the torque needed to rotate the swimming model and the force needed to propel the model forward.
“We found that if the tail propulsion was too weak, the bacteria remain stuck in the gel,” Mohammadigoushki said. “If the force was strong enough it could penetrate the gel. It’s kind of like when you are drilling a screw into a solid wall. If your drill isn’t strong enough and you are not pushing the screw with enough force, it won’t penetrate the wall, but with the right amount of force, it can break through.”
The swimming motions and force that allow H. pylori to move also apply to larger objects, such as earthworms that burrow in the soil, various parasites and more.
“If we understand how the bacteria successfully move to attack our body, we can use that information for whatever we can imagine,” said Kourosh Shoele, an assistant professor in the Department of Mechanical Engineering.
Shoele is part of the multidisciplinary research team and is an expert in computational science. He explained how learning from nature can get a better response from mechanical and biological systems.
“In the future, we can design a micro-robot that can deliver a drug to a particular location in the body, in terms of fighting leukemia and other diseases,” Shoele said.
“Or perhaps we can design tiny robots that use swimming motion and force, like H. pylori, that can dig deep in the sand to explore for water or oil. The possibilities are endless.”
Farshad Nazari, an FSU doctoral student in chemical and biomedical engineering, is working with the two researchers and is the leading author of this paper.
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