Researchers develop an efficient host-vector system for a model archaeon by solving CRISPR-based host-plasmid conflict

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The replicase gene of pRN1, an archaeal cryotic plasmid, carries a DNA segment to be targeted by the host I‐A CRISPR system, and the CRISPR-based host-plasmid conflict is responsible for instability of pRN1‐derived vectors in the archaeon Saccharolobus islandicus REY15A.

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